Máy đếm tế bào tự động RWD
Model: C100/C100SE/C100-Pro Automated Cell Counter
Thương hiệu: RWD
- Cell Analysis Overivew
RWD C100 Automatic Cell Counter Realizes Analysis With Multiple Application Scenarios
Cell Count Analysis: Suitable for counting cells or particles with a diameter of 4-60μm, such as most cell lines, stem cells, primary cells, etc.
Cell Viability Analysis: When testing cell viability in trypan blue mode, after staining, live cells will be circled in green, and dead cells will be circled in red.
Cell Size Analysis: In cell research, cell size is a key feature, which is often measured in cell transfection, drug testing and cell viability.
After counting, it will count the average diameter of the cells in the sample and automatically generate a histogram.
Cell Fluorescence Analysis:
Detect Cell Transfection Efficiency (GFP, RFP):
When transfecting cells with the target gene, the transfection efficiency needs to be evaluated. In general, the transfected plasmid has the sequence encoding the fluorescent protein as the reporter gene, so the successfully transfected cell should be positive for the fluorescent protein. In addition, using a fluorescence microscope or a flow cytometer is a commonly used method for transfection efficiency analysis in laboratories, but both have the disadvantages of complicated operation and high cost. Using the fluorescence analysis function of C100, equipped with the corresponding fluorescence module, can realize the analysis of the expression of GFP, RFP, and other fluorescent proteins, and automatically count them, so as to quickly, simply, and efficiently obtain the positive rate of target gene expression.
Cell Viability Identification (AO/PI Dual Fluorescence Method).
In addition to trypan blue staining, AO/PI dual fluorescence staining has become popular as a more accurate live cell counting method and is widely used for primary cell counting.
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